Laboratory studies of ReGenPRP

Separation and enrichment of thrombocytes with the ReGenPRP-Kit

A. Whole blood
B. Plasma before centrifugation
C. PRP (plasma rich in thrombocytes) after the 2nd centrifugation
D. PPP (Plasma poor in thrombocytes), supernatant after the 2nd centrifugation

Fig. 1 The ReGenPRP prepared according to the standard protocol (see page 16) shows a significant increase in the concentration of thrombocytes

Separation and enrichment in leukocytes, separation and reduction of the number of erythrocytes with the ReGenPRP-Kit

A. Whole blood
B. Plasma before centrifugation
C. PRP (plasma rich in thrombocytes) after the 2nd centrifugation
D. PPP (Plasma poor in thrombocytes), supernatant after the 2nd centrifugation
Fig. 2 : The ReGenPRP prepared according to the standard protocol (see page 16) shows an important increase in the number of leuko-cytes (in blue) and a significant reduction in the number of erythrocytes (in red).


Stability of growth factors and specific plasma proteins retrieved into the ReGenPRP
The preparation of the ReGenPRP (platelet-rich plasma) with the ReGenPRP-Kit is simple and rapid. Often, for organisational or technical reasons, it is necessary to prepare the quantity of the PRP required for the patient’s therapy the day before the operation. This is possible, because the growth factors and the plasma proteins, retrieved into the ReGenPRP, retain their biological activities for at least 20 hours, as long as PRP tubes are conserved at 4°C. Before used, the PRP and the activator are simply warmed for 10 minutes at 37°C. The results of stability tests are shown in figures 11 to 15.

Platelet Derived Growth Factor

Concentration of PDGF aa at 0 hour and after 22 hours at 4°C. Preparation of the ReGenPRP the day before the operation. (Kit ELISA R&D)


Transforming Growth Factor

Concentration of TGF-beta 1 at 0 hour and after 22 hours at 4°C. Preparation of the ReGenPRP the day before the operation. (Kit ELISA R&D)


Epidermal Growth Factor

Concentration of EGF at 0 hour and after 22 hours at 4°C. Preparation of the ReGenPRP the day before the operation. (Kit ELISA R&D)


Vascular Endothelial Growth Factor

Concentration of VEGF at 0 hour and after 22 hours at 4°C. Preparation of the ReGenPRP the day before the operation. (Kit ELISA R&D)


Fibronectin

Concentration of the adhesion protein Fibronectin at 0 hour and after 22 hours at 4°C. Preparation of the ReGenPRP the day before the operation. (Kit ELISA R&D)

 

Concentration of the coagulation markers, D-dimers, in whole blood and in the PRP samples of 10 adult subjects

D-dimers in the whole blood
D-dimers in the PRP

Subjects :
01 - 05 : healthy subjects
06 - 10 : diabetic subjects

The preparation of the ReGenPRP and the materials used do not change the concentration of D-dimers.

 

Laboratory-medical evaluation of PRP prepared with the ReGenPRP-Kit

  • Effective separation and enrichment of the cells necessary for healing (thrombocytes and leukocytes),
  • The stability of the growth factors for at least 20 hours is observed when PRP is stored at 4°C,
  • The efficacy of coagulation factors is preserved.
Prof. Dr. med. D. Hayoz, Divison of Hypertension and Vascular medicine, CHUV, Lausanne.
   
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